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Fig. 6 | Cancer Cell International

Fig. 6

From: Knock down of TIMP-2 by siRNA and CRISPR/Cas9 mediates diverse cellular reprogramming of metastasis and chemosensitivity in ovarian cancer

Fig. 6

Effect of TIMP-2 suppression on migration and invasion. The cell lines studied were gRNA1 (1), gRNA2 (2), CRISPR control (C), parental (P) and pooled siRNA (A + B + C) TIMP-2 suppressed (T2-KD), vector control (C) and parental (P, parental cell line treated with lipofectamine) cells. A, C Migration and B, D invasion were assessed by xCELLigence real-time cell analysis. For assessment of invasion, the electrodes were coated with Matrigel and the bottom of the well contained reduced serum medium (OPTI-MEM). Significance was assessed by linear regression analyses of the slopes and results are shown in the tables next to each graph. Significance obtained using One-way ANOVA and is indicated by ****p < 0.0001

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