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Fig. 1 | Cancer Cell International

Fig. 1

From: Therapeutic effect of a MUC1-specific monoclonal antibody-drug conjugates against pancreatic cancer model

Fig. 1

HzMUC1 antibody binds to MUC1 on the cell surface of pancreatic cancer cells. A Lysates from SW1990, Mia-PaCa-2, PATU-8988, PANC-1, CFPAC-1, and Capan-2 pancreatic cancer cell lines, and hTERT-HPNE (a normal epithelial pancreatic cell line) were resolved by SDS-PAGE and immunoblotted with the commercially available anti-MUC1 N-terminal (NT) antibody and reprobed with anti-β-actin antibody as loading control. B Cell lysates from Capan-2, CFPAC-1, and SW1990 cells were immunoprecipitated with human IgG or HzMUC1 antibody and immunoblotted with the anti-MUC1-NT antibody or anti-MUC1-CT antibody. C HzMUC1 antibody binds to MUC1 protein on the cell surface. Capan-2, CFPAC-1 and SW1990 cells grown on coverslips were washed with PBS, incubated with HzMUC1 antibody (1 µg/well) on ice, and subsequently with Alexa 488-conjugated secondary antibody, and examined with fluorescent (upper panels) and phase contrast (lower panels) confocal microscopy. Scale bars, 20 μm

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