Fig. 1

Clinical data, Morphology, Short tandem repeat (STR) detection and Karyotypic analysis of the ICC-X1 cell line. A1–A3 Show an 8 × 7 cm tumor in the S7 segment of the liver, with limited diffusion, obvious enhancement in the arterial phase, and decreased enhancement in the portal venous phase and delayed phase; A3 A satellite lesion (short arrow) can be seen next to the tumor. A4 There was an 8 × 7 cm gray-white, ductile mass in the section of the surgically resected specimen, and a satellite lesion (short arrow) can be seen next to it. B Clinical and pathological profile of the patient with ICC. C1 Shows an image of day 3 of the primary culture. C2 Shows an image of day 7 of the primary culture. C3 Shows an image of passage 1 of the ICC-X1 cells. C4 Shows an image of passage 5 of the ICC-X1 cells. C5 Shows an image of passage 15 of the ICC-X1 cells. C6 Shows an image of thawed passage 50 of the ICC-X1 cells. Scale bars, 50 μm. D Comparison of genotyping results in tumor specimens obtained from patients, and ICC-X1cells at passage 10. The patient’s tumor specimen and cultured ICC-X1 cells demonstrated differentially typed loci in D21S11, AMEL, D13S317, and PentaD. E The karyotype of the ICC-X1 cell line shows abnormalities in both the number and structure of chromosomes