Fig. 4

The expression of genes encoding key ccRCC secretome proteins correlates with immune infiltration in tumors. A The expressions of SPARC correlates with the presence of endothelial cells in ccRCC tumors. The plots show results of analysis performed with Timer platform (http://timer.comp-genomics.org/). B Top enriched terms for biological processes for genes positively correlating with SPARC in ccRCC tumors. Only genes with r ≥ 0.5 correlations were analyzed. The analysis was performed using ShinyGO 0.76 (http://bioinformatics.sdstate.edu/go/). C The expression of SPARC correlates with key angiogenic regulators in ccRCC tumors. The plots show results of analysis performed with UALCAN (http://ualcan.path.uab.edu/index.html) platform. D SPARC emerges as the top gene correlating with immune infiltration in PanCancer analysis. Volcano plot shows the results of correlation analysis between the expression of 85 genes encoding proteins of ccRCC secretome and the presence of immune cells infiltrating 40 tumor types. SPARC correlations (r ≥ 0.9) are shown with colorful dots. E Representative plots showing top SPARC-CAFs correlations in cancers of breast (BRCA-LumA, BRCA-Her2) and colon (COAD). For detailed data see Additional file 2: Table S10. EPIC, MCPCOUNTER, TIDE: different algorithms utilized by Timer for calculation of immune infiltration