Fig. 6

NEDD8 expression is causally associated with autophagosome formation in OSCC cells. A–C Western blot analyses of the protein levels of Beclin-1, Atg5, LC3-I/II and GAPDH in HSC4 cell variants (A), HSC3 cell variants (B) and HSC4 cells treated with the indicated MLN4924 concentrations for 24 h (C). D–F Western blot analyses for protein levels of Beclin-1, Atg5, LC3-I/II and GAPDH (D) and crystal violet staining (E)/histogram (F) for the results of three independent colony formation assays for vector control HSC3 cells and NEDD8-overexpressing HSC3 cells treated without (untreated, UT) or with 0.1% DMSO (Mock) and indicated 3-MA concentrations for 24 h before irradiation treatment at 4 Gy. The symbol “***” represents statistical significance at p < 0.001