Fig. 4
From: Exosomal MFI2-AS1 sponge miR-107 promotes non-small cell lung cancer progression through NFAT5
MFI2-AS1 as a competing endogenous RNA (ceRNA) for miR-107 in HUVECs. A After nuclear/cytoplasmic separation, MFI2-AS1 is mainly located in the cytoplasmic fraction. U6 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used as controls. B RIP assay was used and qRT-PCR was performed on co-precipitated RNA. Compared with IgG immunoprecipitation, RNA levels were fold enriched in Ago2. C Real-time PCR analysis of 3 candidate miRNAs after co-culture of HUVECs with exo-MFI2-AS1-shRNA. D Association of NSCLC patient survival among 3 candidate miRNAs. E Sequence alignment display and dual-luciferase reporter of the binding site of miR-107 to the MFI2-AS1 region. F The proliferative capacity of HUVECs was assessed by CCK8 assay. G The angiogenic capacity of HUVECs was assessed by in vitro Matrigel tube formation assay. H The cell migration ability of HUVECs was assessed by Transwell migration assay. The data expressed as the mean ± SD. (*P < .05; **P < .01; ***P < .001)