Fig. 5
From: Exosomal MFI2-AS1 sponge miR-107 promotes non-small cell lung cancer progression through NFAT5
MFI2-AS1 promotes angiogenesis by regulating miR-107/NFAT5/AKT signaling pathway in HUVECs. A NFAT5 was discovered as a potential downstream target of miR-107 by using TargetScan, miRWALK and miRDB databases. B Real-time PCR analysis of miR-107 in HUVECs after transfection of miR-107 mimics and miR-107 inhibutor. C Real-time PCR analysis of NFAT5 in HUVECs after transfection of miR-107 mimics and miR-107 inhibutor. D Sequence alignment display of the binding site of miR-107 to the NFAT5 region and dual luciferase reporter. E Real-time PCR analysis of NFAT5 after co-culture of HUVECs with exo-MFI2-AS1-shRNA. F Western blot analysis of indicated proteins in exo-MFI2-AS1-sh HUVECs. G–H The effects of exo-MFI2-AS1 and NFAT5 on migration and angiogenesis were assessed by Transwell and angiogenesis assays. The data expressed as the mean ± SD. (*P < .05; **P < .01; ***P < .001)