Fig. 2

SNHG18 significantly inhibited the proliferation of bladder cancer cells in vivo and in vitro. A, B Effect of SNHG18 on the proliferation of UMUC3 A and J82 B cells, as detected by ATP assays. C, D Effect of SNHG18 on anchorage-independent growth in UMUC3 cells, as detected by soft agar assays. C Representative microscope images and D number of colonies per 10⁴ cells. E, F Effect of SNHG18 on anchorage-independent growth in J82 cells, as detected by soft agar assays. E Representative microscope images and F number of colonies per 10⁴ cells. G, H UMUC3 SNHG18-overexpressing and vector control cells were subcutaneously injected into the right back of nude mice. After 4 weeks, the mice were euthanized, and tumors were surgically removed, weighed, and photographed. I Total RNA was extracted from mouse xenograft tumor tissue, and qPCR was used to detect relative SNHG18 expression in tumor tissues from nude mice. Data are presented as the mean ± SD, *p < 0.05