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Fig. 5 | Cancer Cell International

Fig. 5

From: SNHG18 inhibits bladder cancer cell proliferation by increasing p21 transcription through destabilizing c-Myc protein

Fig. 5

SNHG18 promoted p21 transcription by attenuating c-Myc protein expression. A Dual luciferase reporter assays detected p21 promoter activity in UMUC3 (vector, SNHG18) and J82 (vector, SNHG18) cells. B The mRNA stability of p21 in UMUC3 (vector, SNHG18) was detected. C Potential transcription factor binding sites in the p21 promoter. D Expression levels of potential transcription factors were determined by western blot. E The c-Myc overexpression plasmid was stably transfected into UMUC3 SNHG18-overexpressing cells, and western blotting was used to detect c-Myc and p21 protein levels. F Dual luciferase reporter experiments after c-Myc overexpression were used to detect changes in p21 promoter activity in UMUC3 SNHG18-overexpressing cells. G qPCR to detect p21 mRNA levels in UMUC3 SNHG18-overexpressing cells after restoring c-Myc. H ATP assays were used to detect the proliferation of UMUC3 SNHG18-overexpressing cells after rescuing c-Myc expression

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Cancer Cell International

ISSN: 1475-2867

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