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Fig. 4 | Cancer Cell International

Fig. 4

From: Changes in calpain-2 expression during glioblastoma progression predisposes tumor cells to temozolomide resistance by minimizing DNA damage and p53-dependent apoptosis

Fig. 4

TMZ sensitivity of U251N cell deprived of calpain expression. a Western blot analysis showing the shRNA expression knockdown of either CAPN1 or CAPN2 in U251N cells. We applied two shRNAs (termed #1 and #2) for CAPN1 and CAPN2, respectively. b Cell viability of U251N deprived of either CAPN1 or CAPN2 compared to the shCTRL after 70 µM TMZ administration for 5 days (n = 3, ANOVA and Sidak’s Test). Error bars indicate the standard deviation. c Exemplary fluorescent images showing the propidium iodide (dead cells, red) and Hoechst (all cell nuclei, blue) stainings in U251N shRNA knockdowns. The numbers below the images indicate the percentage of PI positive nuclei in these images

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