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Fig. 5 | Cancer Cell International

Fig. 5

From: Changes in calpain-2 expression during glioblastoma progression predisposes tumor cells to temozolomide resistance by minimizing DNA damage and p53-dependent apoptosis

Fig. 5

TMZ induced apoptotic cell death in U251N. a Fluorescent images of U251N wild type cells after TMZ ± PD administration. Apoptosis was inhibited with Q-VD-OPh, necroptosis with necrostatin-1 (Nec-1). Cell nuclei were stained with Hoechst (blue), apoptotic cells with propidium iodide (red). The numbers below the images indicate the percentage of PI positive cells. b Western blot analysis of cleaved caspase-3 (CASP3) in U251N calpain knockdown cells ± TMZ administration. c Apoptosis induction in U251N cells. 2,5*104 cells were seeded (t = 0) and exposed to the BH3 mimetic ABT-737 and the Mcl-1 inhibitor. After 24 h cells were stained with trypan blue (dead cells) and counted. Error bars show the standard deviation

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Cancer Cell International

ISSN: 1475-2867

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