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Fig. 7 | Cancer Cell International

Fig. 7

From: Changes in calpain-2 expression during glioblastoma progression predisposes tumor cells to temozolomide resistance by minimizing DNA damage and p53-dependent apoptosis

Fig. 7

Neocarzinostatin (NCS) mediated DNA damage induction in U251N. a Experimental procedure of the DNA damage induction. b ɣH2A.X response of U251N and control Hela cells to NCS. U251N calpain knockdown cells were compared to the U251N shCTRL (ANOVA and Sidak’s test). Error bars indicate the standard deviation. c Comet assay results assessing the DNA damage levels (olive moment) after NCS or tbOOH administration (Kruskall-Wallis ranksum test (p < 0.0001) followed by pairwise comparisons using the Wilcoxon rank sum test and Bonferroni correction). d TP53 activity of U251N wild type cells in comparison to T24 (negative control) and Mcf-7 (positive control) cells normalized to 1 µg nuclear extract (NE). Error bars indicate the standard deviation. e TP53 mRNA expression levels in U251N shCTRL and shCAPN2 knockdown cells indicate no difference. f Fluorescent staining for TP53 (AF555, green) and TP53BP1 (AF647, red) in U251N shCTRL and shCAPN2 cells after NCS administration. Cell nuclei are stained with Hoechst (blue). Left panel shows 1000-x magnification with white arrows indicating TP53BP1 “hotspots” outside the nuclei. Right panel shows 400-x magnification of NCS treated and MES control cells

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