Fig. 2

BRF2 knockdown inhibits the invasion and metastasis of liver cancer Huh-7 cells. A The levels of BRF2 mRNA in five types of liver cancer cells were measured by RT-qPCR. B The levels of BRF2 mRNA in Huh-7 cells after lentiviral infection with BRF2 short-hairpin RNA (sh-BRF2) or control short-hairpin RNA (sh-NC) were measured by RT-qPCR. ∗  ∗  ∗ , P < 0.001. C Left: Representative western blot data showing BRF2 levels in Huh-7 cells after lentiviral infection with sh-BRF2 and sh-NC. Right: BRF2 levels are presented as the mean ± standard deviation (SD); n = 3. ∗  ∗  ∗ , P < 0.001 compared with the control group. D Representative western blot data showing E-cadherin and N-cadherin in Huh-7 cells after lentiviral infection with sh-BRF2 and sh-NC. E Invasion and migration of Huh-7 cells after lentiviral infection with sh-BRF2 and sh-NC were measured by wound-healing assay. Left: Representative images at 100 × magnification; scale bar, 100 µm. Right: Quantification of wound-healing assay. WT, Wild type. ∗  ∗  ∗ , P < 0.001, sh-NC vs sh-BRF2 group. F Left: Representative images of transwell migration and invasion assays in Huh-7 cells after lentiviral infection with sh-BRF2 and sh-NC. Magnification, 200 × ; scale bar, 50 µm. Right, Quantification of transwell migration and invasion assays. n = 3. WT Wild type. ∗  ∗  ∗ , P < 0.001. sh-NC vs sh-BRF2 group