Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: BRF2 is mediated by microRNA-409-3p and promotes invasion and metastasis of HCC through the Wnt/β-catenin pathway

Fig. 3

BRF2 is a direct target of miR-409-3p. A Predicted binding site of miR-409-3p in the BRF2 3′ UTR according to TargetScan. B Luciferase reporter assay detecting levels of BRF2 WT 3′ UTR (BRF2-WT) and BRF2 mutant 3′ UTR (BRF2-mut) co-transfected with either an miR-409-3p mimic or a negative control (NC) in 293 T cells and Smmc-7721cells.    , P < 0.01. C, D RT-qPCR measurement of BRF2 mRNA levels after overexpression or depletion of miR-409-3p in Huh-7 cells.      , P < 0.001. E Left: Representative western blots showing BRF2 expression in Huh-7 cells after overexpression or depletion of miR-409-3p in Huh-7 cells. Right: BRF2 levels were quantified and are given as the mean ± SD; n = 3.      , P < 0.001. F Left: Representative images of Huh-7 cells after overexpression or depletion of miR-409-3p for a wound-healing assay. Magnification, 200 × ; scale bar, 50 µm. Right: Quantification of the wound-healing assay. n = 3.      , P < 0.001. G Left: Images of transwell migration and invasion assays in Huh-7 cells after overexpression or depletion of miR-409-3p. Magnification, 200 × . scale bar, 50 µm. Right, Quantification of transwell migration and invasion assays. n = 3.      , P < 0.001

Back to article page