Fig. 2

Over-expression of PCK1 inhibits CRC cell proliferation in vitro and in vivo. A Viability of SW480 and RKO cells in the Ctrl, PCK1-OE, and PCK1-sh groups over time (24, 48, 72, 96 and 120 h), measured by CCK8 assay (450 nm absorbance). B Colony formation of SW480 and RKO cell lines in the Ctrl, PCK1-OE, and PCK1-sh groups at 15 days. C Tumor volume and weight were measured 24 days after tumor cells were injected into BALB/c- nude mice. *P < 0.05, **P < 0.01, and ***P < 0.001