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Fig. 3 | Cancer Cell International

Fig. 3

From: PCK1 activates oncogenic autophagy via down-regulation Serine phosphorylation of UBAP2L and antagonizes colorectal cancer growth

Fig. 3

PCK1 affects colorectal cancer cell autophagy. A and B Western blot analysis of P62, ATG5, and LC3B protein expression in SW480 (A) and RKO (B) cells transfected with PCK1-OE or PCK1-Ctrl for 12, 24, and 48 h. C Western blot analysis of P62, ATG5, and LC3B protein expression in SW480 cells transfected with PCK1-sh or PCK1-Ctrl for 12, 24, and 48 h. Actin was used as a control. D and E Immunofluorescence dots in SW480 (D) and RKO (E) cells transfected with PCK1-OE or PCK1-Ctrl and GFP-RFP-LC3 for 24 h. F Immunofluorescence dots in SW480 cells transfected with PCK1-sh or PCK1-Ctrl and GFP-RFP-LC3 for 24 h. G and H) Electron microscope images showing autophagic vesicles in SW480 (G) and RKO (H) cells transfected with PCK1-OE or PCK1-Ctrl for 24 h. I Electron microscope images showing autophagic vesicles in SW480 cells transfected with PCK1-sh or PCK1-Ctrl for 24 h. *P < 0.05, **P < 0.01, and ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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