Fig. 5
From: A novel feedback regulated loop of circRRM2-IGF2BP1-MYC promotes breast cancer metastasis
circRRM2 serves as a miR-27b-3p or miR-31-5p sponge in BC cells. A RIP assays using the AGO2 antibody for circRRM2 immunoprecipitation. B The seed sequences between circRRM2 and miR-27b-3p/miR-31-5p in the RNA22 database. qRT-PCR results of miR-27b-3p/miR-31-5p after circRRM2 knockdown in MDA-MB-231 (C) or BT-549 (D) cells. E The co-localization between circRRM2 and miR-27b-3p was observed in the cytoplasm using the FISH experiment. The prognostic value of miR-27b-3p (F)/miR-31-5p (G) in the TCGA-BRCA dataset was tested using the Kaplan–Meier analysis. *P < 0.05, **P < 0.01