Fig. 1

SETD8 declines ferroptosis in pancreatic cancer cells. A, B The shRNAs against SETD8 plasmid were transfected into SW1990 cell line. Plasmid overexpressing SETD8 was transfected into Mia PaCa-2 cell line. Western blot analysis and qPCR analysis were performed to exam SETD8 protein and mRNA levels, respectively. C The MDA was detected in SETD8-silenced SW1990 cell line and SETD8-overexpressed Mia PaCa-2 cell line. The results showed that SETD8 could inhibit the expression of MDA. D The GSH/GSSG ratio was detected in SETD8-silenced SW1990 cell line and SETD8-overexpressed Mia PaCa-2 cell line. It was found that silencing SETD8 could decrease the GSH/GSSG ratio and overexpression of SETD8 could increase the GSH/GSSG ratio. E Cell viability was detected in the SETD8-silenced SW1990 cell line in the presence or absence of 2 μmol/l Fer and the SETD8-overexpressed Mia PaCa-2 cell line in the presence or absence of 2 μmol/l RSL3. The results showed that the cell viability decreased by SETD8 knockdown could be reversed by the ferroptosis inhibitor Fer and the cell viability increased by SETD8 overexpression could be reversed by the ferroptosis inducer RSL3. F Flow cytometry analyzed the fluorescence of BODIPY581/591C11 (lower) and the relative content was calculated (upper). The results showed that SETD8 could reduce the level of intracellular lipid peroxidation. Two tailed unpaired Student t-test was used in the above experiments. *P < 0.05; **P < 0.01