Fig. 3From: SETD8 inhibits ferroptosis in pancreatic cancer by inhibiting the expression of RRADRRAD inhibits the proliferation of pancreatic cancer cells and is associated with the prognosis of patients with pancreatic cancer. A, B Western blot analysis and qPCR analysis were performed to exam RRAD protein and mRNA levels, respectively. The overexpression of RRAD was confirmed in Mia PaCa-2 cell line. C CCK-8 assay showed that overexpression of RRAD decreased the proliferation of pancreatic cancer cells. D Colony formation assay showed that overexpression of RRAD reduced the proliferation of pancreatic cancer cells. E Representative images of IHC staining of RRAD in PDAC tumour tissues and paired adjacent normal tissues. The results showed that RRAD was underexpressed in pancreatic cancer. F lHC analysis further confirmed that RRAD protein levels were lower in cancer tissues. wilcoxon rank test P = 0.0015. G Kaplan–Meier survival rate analysis for PDAC patients showed high RRAD expression was associated with longer over survival. *P < 0.05; **P < 0.01Back to article page