Fig. 5
From: SETD8 inhibits ferroptosis in pancreatic cancer by inhibiting the expression of RRAD
SETD8 influences the occurrence of ferroptosis in pancreatic cancer through RRAD. A, B qPCR and Western blot assay confirmed the efficiency of shRNAs targeting SETD8, RRAD or both in the SW1990 cell line and the overexpression efficiency of SETD8, RRAD or both in the Mia PaCa-2 cell line. C Cell viability was detected in SETD8-, RRAD- or both-silenced SW1990 cell lines and in SETD8-, RRAD- or both-overexpressed Mia PaCa-2 cell line. The results showed that overexpression of SETD8 enhanced cell viability, which was reversed by overexpression of RRAD. The increase in cancer cell activity caused by silencing RRAD expression was reversed by silencing SETD8 expression. D Flow cytometry analyzed the fluorescence of BODIPY581/591C11 (lower) and the relative content was calculated (upper). The results showed that SETD8 knockout induced elevated lipid peroxidation in cancer cells, which could be reversed by silencing RRAD expression. The elevated lipid peroxidation induced by RRAD overexpression was reversed by overexpression of SETD8. *P < 0.05; **P < 0.01