Fig. 1

Detection of expression of ICL, hsa-miR19-3p, NKRF and phosphorylation of NF-κB-p65 in LC tissues of LC patients with and without COPD and their correlation analysis. (A) The relative expression of ICL were detected in LC tissues by RT-qPCR. (B) Detection of ICL in LC tissues by FISH. (C) The relative levels of hsa-miR19-3p and NKRF mRNA were detected in LC tissues by RT-qPCR. (D) Phosphorylation of NF- κB-p65 and expression of NKRF protein in LC tissues were detected by western blotting. (E) Spearman correlation analysis between phosphorylation NF-κB-p65 and hsa-miR19-3p, hsa-miR19-3p and NKRF protein in LC tissues. RT-qPCR was performed using U6 and β-actin as references, respectively, and the 2^−ΔCt method was used for analysis of inter-group difference. For western blotting, β-actin was used as the reference. ^**p < 0.01 and ^*p < 0.05. All data are expressed as the mean ± SD (sample size, n = 12)