Fig. 3

Analysis of the effects of ICL on proliferation, invasion and migration in LCs and C-LCs. (A) CCK-8 assay. The y-coordinate represents the absorbance of samples at 450 nm. (B) Cell invasion assay. Cell invasion assay was performed by trans-well method. The cells passing through the membrane were fixed with ice-cold methanol followed by hoechst33342 staining (Left). The number of cells was counted using light microscopy, and the data are presented graphically (Right). (C) Wound healing assay on the migration of LCs and C-LCs infected with Lv-control or Lv-ICL. The calculation formula of wound healing percentage (relative width) was as follows: (the width of wound at 0 h- the width of wound at 24 h)/the width of wound at 0 h. ** p < 0.01, * p < 0.05. The tests were carried out on three biological triplicates, and data are expressed as the mean ± SD.