Fig. 3

LncRNA SATB2-AS1 repressed HCC growth and metastasis in vivo. Huh7 cells transfected with negative vector or SATB2-AS1 overexpression plasmids were subjected to the subcutaneous xenografted tumor experiment on nude mice. A, C: The nude mice were sacrificed after 5 weeks, and the subcutaneous tumor nodules were removed. The volume (B) and weight (C) of tumor nodules were calculated. D. The SATB2-AS1 expression in vivo was monitored by RT-PCR. E: IHC was implemented to examine the number of Ki67-positive cells in Huh7. Scale bar = 50 μm. F: Western blot was conducted for detecting apoptosis-related proteins (including Bad, bcl2, Bax, and cleaved Caspase3) levels in the tumor tissues. G. HE staining was applied to test lung metastasis of HCC, and the number of lung metastasis was counted. H: The profiles of E-cadherin, Vimentin, and N-cadherin were compared by WB. I: IHC was applied to calculate positive cells for E-cadherin and Vimentin. Scale bar = 50 μm. ns indicates P > 0.05, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001. N = 5