Fig. 4

LncRNA SATB2-AS1 facilitated GRIM-19 and inactivated the STAT3/HIF-1α pathway. The SATB2-AS1 overexpression model was established in Huh7 and HCCLM3 cells. A, B: The expression of GRIM-19 mRNA and the GRIM-19/STAT3/HIF-1α pathway were measured by RT-PCR (A) and WB (B), respectively. C: Cellular immunofluorescence was conducted to examine the fluorescence intensity of GRIM-19 (marked by green fluorescence). Scale bar = 50 μm. D: IHC was applied to determine the expression of GRIM-19 in the tumor tissues. Scale bar = 50 μm. IHC score was counted. E: WB was implemented to examine the GRIM-19/STAT3/HIF-1α pathway expression in the tumor tissues. *** indicates P < 0.001. N = 3