Fig. 7

MiR-3678-3p accelerated the malignant phenotypes of HCC. The miR-3678-3p mimic was transfected in Huh7 cells. A: RT-PCR detected the transfection effect of miR-3678-3p. B, C: Colony formation experiment and EdU staining were applied to examine cell proliferation. Scale bar = 50 μm. D: Western blot was conducted for detecting cell cycle-related proteins (including p21, Cyclin D1, CDK4, Cyclin E1, and CDK2) levels in HCC cells. E. Flow cytometry (FCM) was adopted to monitor the HCC cell apoptosis rate. F: Western blot was conducted for detecting apoptosis-related proteins (including Bad, bcl2, Bax, cleaved Caspase3) levels in HCC cells. G: Huh7 cell migration and invasion were monitored by Transwell assay. Scale bar = 100 μm. H: WB was implemented to test the profiles of EMT-related proteins. I: WB was conducted to measure the GRIM-19/STAT3/HIF-1α pathway level in Huh7 cells. ** indicates P < 0.01, *** indicates P < 0.001. N = 3