Fig. 8

SATB2-AS1 impeded the carcinogenic effect mediated by miR-3678-3p. SATB2-AS1 was overexpressed in Huh7 cells transfected with miR-3678-3p mimics. A, B: Colony formation experiment and EdU staining were applied to test cell proliferation. Scale bar = 50 μm. C: Western blot was conducted for detecting cell cycle-related proteins (including p21, Cyclin D1, CDK4, Cyclin E1, and CDK2) levels in HCC cells. D. Flow cytometry (FCM) was adopted to monitor the HCC cell apoptosis rate. E: Western blot was conducted for detecting apoptosis-related proteins (including Bad, bcl2, Bax, and cleaved Caspase3) levels in HCC cells. F: Huh7 cell migration and invasion were monitored by Transwell assay. Scale bar = 100 μm. G. The levels of EMT-related proteins were compared by WB. H: WB was performed to test the expression of the GRIM-19/STAT3/HIF-1α pathway in Huh7 cells. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001. N = 3