Fig. 3

OSSC lines were incubated with EGF and S1PR2 treatments for 48 h. (A) Representative images of transwell invasion assay according to the different treatments, captured using fluorescent microscopy. (B) EGF increased invasion of all three cell lines. These effects decreased when S1PR2 was not activated. However, when S1PR2 was activated combined with EGF treatment, invasion did not increase. (Scale bar represents 200 μm. N = 30; ANOVA followed by post-hoc Tukey tests, * = p < 0.05, ** = p < 0.01.)