Fig. 5

The function validation by down-regulated the AUP1 expression. A The protein expression of AUP1 revealed a marked increase compared to the normal control. B, C When knowing down the AUP1 gene, its mRNA expression efficiently downregulated in both proteins and transcriptome levels. D Several markers relevant to invasiveness, ROS, and autophagy were examined, including N-cadherin, Nrf2, mtFTA, p62, LC3B1, and LC3BII. E We also observed the cytosolic ROS and F migration ability after being supplemented with siAUP1; however, the results are inconspicuous. G Then, we checked the proliferation markers, including EGFR and PCNA, and the results showed a marked decrease in the U87MG cells. H But the changes in the cell cycle in U87MG, LNZ308, and GBM8401 were not obvious. I, J In the BrdU analyses of U87MG, the proportion of the cells in the S phase revealed significantly decreased when supplemented with siAUP1. Bars, mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.005