Fig. 1

Accumulation of cholesterol reduces sensitivity to cisplatin in lung cancer cell lines

(A) The IC50 of cisplatin in H1373, H1975, H441, and A549 cell lines treated with PPD or DMSO. (B) Colony formation assay in H1373, H1975, H441, and A549 cell lines treated with PPD or DMSO. (C) The IC50 of cisplatin in A549/DDP cell lines treated with PPD, CHOL, or DMSO. (D) ELISA test kit to detect cholesterol levels (TC) in A549/DDP cell lines treated with PPD, CHOL, or DMSO. (E) The cholesterol accumulation-related marker genes were detected. by qRT-PCR in A549/DDP cell lines treated with PPD (Gray), CHOL (Purple), or PPD (Blue). The expression level was displayed from low (blue) to high (red). PPD Pseudoprotodioscin, a cholesterol inhibitor, CHOL cholesterol. The experiments were repeated three times. For comparisons, a Student’s t-test was performed. ^*P < 0.05, and ^**P < 0.01