Fig. 2From: SIAH1 ubiquitination-modified HMGCR inhibits lung cancer progression and promotes drug sensitivity through cholesterol synthesisCholesterol biosynthesis induced by HMGCR inhibits cisplatin sensitivity in lung adenocarcinoma cell lines(A) The relative mRNA level of HMGCR in normal cells (BEAS-2B) and lung cancer cell lines (H1373, H1975, H441, and A549). (B) The protein level of HMGCR in normal cells (BEAS-2B) and lung cancer cell lines (H1373, H1975, H441, and A549). (C) The protein level of HMGCR in A549 and A549/DDP cells. (D) ELISA test kit was to detect cholesterol levels with or without cholesterol in the A549/DDP cell lines with knockout HMGCR. (E) The protein level of HMGCR with or without cholesterol in the A549/DDP cell lines with knockout HMGCR. (F) The IC50 of cisplatin with or without cholesterol in the A549/DDP cell lines with knockout HMGCR. The experiments were repeated three times. The experiments were repeated three times. For comparisons, a Student’s t-test was performed. *P < 0.05, and **P < 0.01Back to article page