Fig. 3

Effects of HMGCR overexpression or knockout on the proliferation of lung cancer cell lines

(A-B) Detection of relative mRNA and protein levels of HMGCR in A549 and A549/DDP cell lines overexpressing or knocking down HMGCR. (C) CCK8 assay was to evaluate the proliferation in the constructed A549 and A549/DDP cell lines overexpressing or knocking out HMGCR. (D) Colony formation assay in the constructed A549 and A549/DDP cell lines stable overexpressing or knocking out HMGCR was to test cell proliferation. (E) Transwell assay in the constructed A549 and A549/DDP cell lines stable overexpressing or knocking out HMGCR was to evaluate the invasion ability. (F-G) The relative mRNA and protein levels of ABCB1, ABCB4, ABCG1, and ABCG2 in the constructed A549 cell lines overexpressing or knocking out HMGCR. The experiments were repeated three times. For comparisons, a Student’s t-test was performed. ^*P < 0.05, and ^**/##P < 0.01