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Fig. 5 | Cancer Cell International

Fig. 5

From: SIAH1 ubiquitination-modified HMGCR inhibits lung cancer progression and promotes drug sensitivity through cholesterol synthesis

Fig. 5

SIAH1 enhances cisplatin sensitivity through HMGCR inhibition of efflux protein activity to inhibit cell proliferation migration invasion

(A) The IC50 of cisplatin in A549/DDP stable cell lines overexpressing SIAH1 with or without cholesterol. (B) The IC50 of cisplatin in the constructed A549/DDP cell lines knocked down SIAH1 and/or HMGCR. (C-D) The relative mRNA and protein levels of SIAH1, HMGCR, and ABCB1 in the stable cell lines of A549/DDP with SIAH1 and/or HMGCR knockdown. (E) CCK8 assay was to evaluate the proliferation in the constructed A549/DDP cell lines with SIAH1 and/or HMGCR knockdown. (F) Colony formation assay in the constructed A549/DDP cell lines knocked down SIAH1 and/or HMGCR. (G) Transwell assay was to evaluate the invasion ability in the constructed A549/DDP cell lines with SIAH1 and/or HMGCR knockdown. The experiments were repeated three times. For comparisons, Student’s t test was performed. */#P < 0.05, and **/##/^^P < 0.01

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Cancer Cell International

ISSN: 1475-2867

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