Fig. 6

SIAH1 blocks tumor development by enhancing drug sensitivity in vivo through inhibition of cholesterol synthesis by HMGCR

(A) Tumor volume curve of nude mice under SIAH1 overexpression and/or DDP treatment. (B) Detection of TC content in the presence of SIAH1 overexpression and/or DDP treatment. (C) The relative protein levels of SIAH1, HMGCR, and ABCB1 were measured under overexpression of SIAH1 and/or DDP treatment conditions. (D) IHC detection of SIAH1, HMGCR, and ABCB1 protein expression under overexpression of SIAH1 and/or DDP treatment conditions. And representative images of Ki-67 staining and TUNEL analysis in tumors treated. (E) Tumor volume curve of nude mice under SIAH1 or HMGCR depletion. (F) Detection of TC content under SIAH1 or HMGCR depletion. (G) The relative protein levels of SIAH1, HMGCR, and ABCB1 were measured under SIAH1 or HMGCR depletion. (H) IHC detection of SIAH1, HMGCR, and ABCB1 protein expression under SIAH1 or HMGCR depletion. And representative images of Ki-67 staining and TUNEL analysis in tumors treated. The experiments were repeated three times. For comparisons, Student’s t test was performed. ^*P < 0.05, and ^{**/##/^^}P < 0.01