Fig. 3

MiR-3180 inhibits the 3′-UTR activity of CD36 and SCD1. a The mRNA and protein expression of CD36 and SCD1 in HepG2 and MHCC-97H cells transfected with scramble or miR-3180 inhibitor using RT–qPCR and immunoblotting analysis. β-actin was used as a loading control for immunoblotting. b The mRNA and protein expression of CD36 and SCD1 in HepG2 and MHCC-97H cells transfected with NC or miR-3180 mimics using RT–qPCR and immunoblotting analysis. β-actin was used as a loading control for immunoblotting. c, d Luciferase reporter analysis of wild-type (WT) and mutated (Mut) 3′-UTR activity of SCD1 (c) or CD36 (d) in HepG2 and MHCC-97H cells. The putative miR-3180 target sequences in the CD36 and SCD1 3′-UTRs are shown in the top panel. The data shown are presented as the mean ± SD of triplicate measurements with similar results. **p < 0.01