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Fig. 4 | Cancer Cell International

Fig. 4

From: MiR-3180 inhibits hepatocellular carcinoma growth and metastasis by targeting lipid synthesis and uptake

Fig. 4

MiR-3180 suppresses lipid content in a CD36- and SCD1-dependent manner. a Flow cytometry analysis of lipid content in HepG2 cells transfected with miR-3180 and the indicated expression vectors or negative control. The relative lipid content is shown in the middle panel, and the expression of miR-3180, SCD1 and CD36 is shown in the right panel. b Oil red O staining of lipid content in HepG2 cells transfected as in (a). Scale bar: 20 μm. c The relative triglyceride and cholesterol levels in HepG2 cells transfected as in (a). d Flow cytometry analysis of lipid content in HepG2 cells transfected with the indicated vectors. The relative lipid content is shown in the right panel. e Oil red O staining of lipid content in HepG2 cells transfected as in (d). Scale bar: 20 μm. f RT–qPCR and immunoblotting analysis of miR-3180, CD36 and SCD1 expression in HepG2 cells transfected as in (d). G Relative triglyceride and cholesterol levels in HepG2 cells transfected as in (d). The data shown are presented as the mean ± SD of triplicate measurements with similar results. **p < 0.01

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