Fig. 4

Effect of Par3 on AJC marker expression in OSCC cells. a Western blot of protein levels (left) and quantification (right) of AJC markers in OSCC cell lines treated with siRNA-Par3 or control siRNA. The experiments were performed in triplicates. b Localization of Par3 (Green) and TJP1 (Red) in SCC-25 cell line treated with siRNA-Par3 or control siRNA as analyzed by IF staining. Scale bars: 20 μm. The experiments were performed in triplicates. c Interaction between TJP1 and Par3 in SCC-25 cell line treated with siRNA-Par3 or control siRNA. Red spots represent PLA positive signals. Scale bars: 20 μm. The experiments were performed in triplicates. d Proliferation ability of OSCC cells treated with siRNA-Par3 or control siRNA as analyzed by the EdU assay. Green colored cells are EdU-positive cells. The nuclei were stained with DAPI (blue). Scale bars: 20 μm. The experiments were performed at least thrice. e Representative images (left) and numbers (right) of migrated and invaded OSCC cells treated with siRNA-Par3 or control siRNA. The experiments were performed at least thrice. Scale bars: 100 μm. f Activities (left) and fold intensities (right) of MMP-9 and MMP-2 in OSCC cells treated with siRNA-Par3 or control siRNA as analyzed by gelatin zymography. *P < 0.05 vs. control siRNA. The experiments were performed in triplicates. Cont: control siRNA; siR-Par3: siRNA-Par3; Hyp: hypoxia; PLA+: PLA positive signals in OSCC cells; EdU+: EdU-positive cells