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Fig. 4 | Cancer Cell International

Fig. 4

From: Hyperglycemia and O-GlcNAc transferase activity drive a cancer stem cell pathway in triple-negative breast cancer

Fig. 4

Glucose effects of TET1 activation and OGT inhibition in TNBC lines. a Comparison of total cellular TET activity using a 5-hydroxymethyl cytosine (5hmC) assay from TNBC cells grown in low vs. high glucose. MDA-MB-231 cells were cultured in low glucose (1.0 g/L) or high glucose (4.5 g/L) media for 3 days prior to nuclear extraction and 5hmC analysis. Bars indicate SEM of 3 technical replicates per condition, with 3 biological replicates in MDA-MB-231 cells. b Comparison of TARDBP mRNA levels by qRT-PCR between high and low glucose conditions. Media containing high glucose (4.5 g/L) was added for 24 h following growth in low glucose media (1.0 g/L). b TARDBP mRNA levels in OSMI-4 treated (10 µM, 24 h) TNBC cells in high glucose conditions were measured by qRT-PCR, normalized to non-treated controls. Bars indicate SEM of 3 biological replicates, with 3 technical replicates for each cell line. c Comparison of the average OGT inhibition data between low (see Fig. 2k) and high glucose in three TNBC cell lines (HCC70, MDA-MB-468, and MDA-MB-231). d Western blot analysis of TARDBP with OSMI-4 treatment in high glucose media (N = 2, representative blot shown)

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