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Fig. 6 | Cancer Cell International

Fig. 6

From: Hyperglycemia and O-GlcNAc transferase activity drive a cancer stem cell pathway in triple-negative breast cancer

Fig. 6

Impact of OGT inhibition on cancer stem cell maintenance in TNBC cells. a Hypothesized entry point for OGT to our published CSC expansion pathway [13]. b Fluorescence Activated Cell Sorting (FACS) analysis for triple stem cell marker expression under OGT inhibition. The CD44 + population was gated and analyzed further for CD133 and EpCam. Upper right quadrant indicates “triple positive” CSC cell populations. 468_GFP indicates control transfection cells (green fluorescent protein overexpression) and 468_MBD2v2-OE indicates stable MBD2_v2 overexpression as “rescue” construct. Representative data shown (full data and gating strategies shown in Additional file 1: Online Resource 5). c Analysis of FACS data. Lines indicate SEM, N = 3. OSMI treatment duration was 48 h prior to analysis, in low glucose media

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Cancer Cell International

ISSN: 1475-2867

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