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Table 3 Isolation of lymphocyte-lymphocyte fusion products by velocity sedimentation, mean ± standard deviation.

From: Cell fusion in tumor progression: the isolation of cell fusion products by physical methods

Cell isolation by velocity sedimentation

Mononucleated cells (%)

Bi-tri nucleated cells (%)

Polynucleated cells (%)

Fractions

I

0.44 ± 0.75

0.48 ± 0.63

98.88 ± 1.01

 

II

0.21 ± 0.20

5.09 ± 0.87

95.97 ± 0.67

 

III

3.81 ± 0.83

95.94 ± 0.82

0.33 ± 0.72

 

IV

87.06 ± 0.85

13.16 ± 0.85

0.2 ± 0.11

  1. 1000 cells were counted in phase contrast microscopy and in fluorescent microscopy using a filter set appropiate for both emission wavelenghts (CMFDA and CMTMR). (Data from 10 experiments.)