Figure 8

Effect of benzothiazole based compounds on ERK and MAPK proteins that play vital role in cell proliferation. HepG2 cells were treated with compounds YM, A3 and A4 for 24 h. The total lysates were harvested and analysed by immunoblotting with anti-ERK, phosphorylated ERK (Thr 202/Tyr 204), anti-p38 MAPK, phosphorylated form of p38 MAPK (Thr 180/Tyr 182) anti- bodies. YM serves as positive control. β - actin was used as loading control. Western blots were representative of three independent experiments. The expression of phosphorylated ERK and MAPK were found to be down regulated. C: represents control untreated cells. The molecular weights of ERK1/2, Phospho ERK1/2, p38MAPK and phosphop38MAPK were 42/44 KDa, 42/44 KDa, 43 KDa and 43 KDa respectively. The western blot analysis was repeated thrice and densitometric analysis was done. The results were statistically significant with p < 0.001 for A3 and A4 with regard to ERK1/2 phosphorylation and p < 0.05, p < 0.001 for p38 MAPK phosphorylation for A3 and A4 respectively.