Figure 2

NP effect on nuclear NFκB amount. Nuclear extracts blots. (A) PC-3 cells, from left to right: baseline; ET-1 incubations (100 nM) x 30 min, 45 min and 60 min; BQ-123 (1 μM x 30 min) and ET-1 (100 nM x 60 min); pre-incubation with bortezomib (1 μM x 30 min) followed by co-stimulation with ET-1 (100 nM) x 30 min, 45 min and 60 min; pre-incubations with BAY 11–7082 (20 μM x 16 h) and wedelolactone (50 μM x 90 min) followed by ET-1 incubations (100 nM x 1 h). (B) PC-3 cells, from left to right: baseline; BBS incubations (100 nM) x 30 min, 45 min and 60 min; RC-3095 (10 nM x 1 h) and BBS (100 nM x 1 h); pre-incubation with bortezomib (1 μM x 30 min) followed by co-stimulation with BBS (100 nM) x 30 min, 45 min and 60 min; pre-incubations with BAY 11–7082 (20 μM x 16 h) and wedelolactone (50 μM x 90 min) followed by BBS incubations (100 nM x 1 h). (C, D) Summary histograms of mean relative band densities for (A) and (B) normalized to actin expressed as a percentage of baseline (*p < 0.05 versus baseline, ^#p < 0.05 between conditions as indicated by horizontal lines).