Figure 3

NP effect on NFκB-DNA binding. EMSA. (A) From left to right: PC-3 cells, baseline; PC-3 cells, ET-1 incubation (100 nM x 45 min); PC-3 cells, pre-incubations with BQ-123 (1 μM x 30 min), bortezomib (1 μM x 60 min), wedelolactone (50 μM x 90 min) and BAY 11–7082 (20 μM x 16 h) followed by ET-1 incubation (100 nM x 45 min); LnCaP cells, baseline; LnCaP cells, ET-1 incubation (100 nM x 45 min); HeLa cells, infection with Sentai paramyxovirus (Cantell strain) for 6 h. (B) From left to right: PC-3 cells, BBS incubations (100 nM) x 30 min, 45 min and 60 min; pre-incubation with RC-3095 (10 nM x 1 h), wedelolactone (50 μM x 90 min), BAY 11–7082 (20 μM x 16 h) and bortezomib (1 μM x 60 min) followed by BBS incubations (100 nM x 1 h); HeLa cells, rhTNF-α incubation (10 μM x 1 h). (C) From left to right: PC-3 cells, baseline; ET-1 incubations (100 nM) x 45 min and 60 min; BBS incubations (100 nM) x 45 min and 60 min. LnCaP cells, baseline; ET-1 incubations (100 nM) x 45 min and 60 min; BBS incubations (100 nM) x 45 min and 60 min.