Figure 4

NP effect on cellular IκBα amount. Total cell lysate blots. (A) PC-3 cells, from left to right: baseline; ET-1 incubations (100 nM) x 30 min, 45 min and 60 min; pre-incubations with BQ-123 (1 μM x 30 min), wedelolactone (50 μM x 90 min) and BAY 11–7082 (20 μM x 16 h) followed by ET-1 incubation (100 nM x 45 min). (B) PC-3 cells, from left to right: baseline; pre-incubation with bortezomib (1 μM x 30 min) followed by co-stimulation with ET-1 (100 nM) x 45 min and 60 min; ET-1 incubations (100 nM) x 30 min, 45 min and 60 min. (Different parts of the same gel). (C) PC-3 cells, from left to right: baseline; BBS incubations (100 nM) x 30 min, 45 min and 60 min; pre-incubations with RC-3095 (10 nM x 1 h), wedelolactone (50 μM x 90 min) and BAY 11–7082 (20 μM x 16 h) followed by BBS incubation (100 nM x 45 min); pre-incubations with RC-3095 (10 nM x 1 h), wedelolactone (50 μM x 90 min) and BAY 11–7082 (20 μM x 16 h) followed by BBS incubation (100 nM x 45 min). (D) PC-3 cells, from left to right: baseline; pre-incubation with bortezomib (1 μM x 30 min) followed by co-stimulation with BBS (100 nM) x 45 min and 60 min; BBS incubations (100 nM) x 30 min, 45 min and 60 min. (Different parts of the same gel). (E-H) Summary histograms of mean relative band densities for (A-D) normalized to actin expressed as a percentage of baseline (*p < 0.05 versus baseline, ^#p < 0.05 between conditions as indicated by horizontal lines).