Figure 5

NP effect on NFκB subcellular localisation and cellular NFκB, IκBα amount. NFκB expression by ICC and nuclear extract blots. IκBα expression by total cell lysate blots. (A) From left to right: LnCaP cells, baseline; LnCaP cells, ET-1 (100 nM x 45 min); LnCaP cells, BQ-123 (1 μM x 30 min) and ET-1 (100 nM x 45 min); LnCaP cells, BBS (100 nM x 1 h); LnCaP cells, RC-3095 (10 nM x 1 h) and BBS (100 nM x 45 min). (B) From left to right: PC-3 cells, baseline; LnCaP cells, baseline; LnCaP cells, ET-1 incubations (100 nM) x 45 min and 60 min; BBS incubations (100 nM) x 45 min and 60 min. (C) From left to right: PC-3 cells, baseline; LnCaP cells, baseline; ET-1 incubations (100 nM) x 30 min, 45 min and 60 min; BBS incubations (100 nM) x 30 min, 45 min and 60 min.