Figure 8

NP time-dependent effect on 20 S proteasomal activity (RFU/μg) in PC-3 cells. (A) From left to right: PC-3 cells, baseline; time series of ET-1 incubations (80 nM) x 30 min, 45 min and 60 min; time series of ET-1 incubations (100nM) x 30 min, 45 min and 60 min. (B) From left to right: PC-3 cells, baseline; time series of BBS incubations (40 nM) x 30 min, 45 min and 60 min; time series of BBS incubations (100nM) x 30 min, 45 min and 60 min. Data represent the mean (± SEM) of three independent experiments performed in triplicate and are expressed as percent of the initial value of proteasome activity (*p < 0.05 versus baseline, #p < 0.05 between conditions as indicated by horizontal lines).