Figure 2
From: Lycorine induces cell-cycle arrest in the G0/G1 phase in K562 cells via HDAC inhibition
Effects of lycorine on the activity of HDACs in K562 cells. Briefly, nuclear proteins were extracted from K562 cells treated with different concentrations of lycorine (2.5 or 5.0 μM) or without lycorine for 24 h. About 50 μg of nuclear protein from each group was added to a 96-well tissue culture plate and HDAC enzymatic activities in the cell nucleus was determined according to the HDAC colorimetric assay kit instructions. Values are expressed as the percentage of HDAC activities relative to untreated cell extracts. Results are presented as mean ± S.D. (n = 3, three independent experiments). Asterisks (*) indicate significant difference (P < 0.05) compared with the control group.