Figure 1

Overexpression of p65 increases NF-κB transcriptional activity. (A) Cells were transfected with p65cDNA or empty vector and treated with celecoxib (80 μM) for 12 h. (B) NF-κB DNA binding activity was evaluated by EMSA in cells transfected with p65cDNA or empty vector. (C) NF-κB DNA binding activity was evaluated by luciferase reporter gene assay in cells transfected with p65cDNA or empty vector. *P < 0.05, significantly different from empty vector transfected cells. #P < 0.05, significantly different from empty vector transfected cells after celecoxib treatment.