Figure 6
From: miR-125b develops chemoresistance in Ewing sarcoma/primitive neuroectodermal tumor
The effects of miR-125b on the resistance of EWS cells to etoposide and vincristine. A) miR-125b was stably knocked down in Dox-resistant EWS cells. The cells were seeded at 2 × 103 cells/well in 96 well plates, cultured for 12 h, and then treated with various concentrations of vincristine or etoposide for an additional 48 h. The cell viability was determined by the CellTiter-GloTM Luminescent Cell Viability Assay The data represent the means of three separate experiments. The results are the means ± SD. *, P < 0.05. B) Cells were seeded at 2 × 103 cells/well in 96 well plates 36 h after transfection with 100 nM control or has-miR-125b miRNA Precursor. The cells were treated with various concentrations of vincristine or etoposide for an additional 48 h. The cell viability was determined by the CellTiter-GloTM Luminescent Cell Viability Assay. The data represent the means of three separate experiments. The results are the means ± SD. *, P < 0.05. C) The drug sensitivity of the EWS clones to various anticancer agents. IC50 showed drug concentrations that inhibited cell survival by 50%.