Figure 2
From: TWIST1, A novel androgen-regulated gene, is a target for NKX3-1 in prostate cancer cells
AR mediates the effect of androgen on TWIST1 expression. LNCaP cells cultured in RPMI medium containing 10% FCS were transfected with 100 nM of siRNA against AR (AR) or a non-silencing control (ctr.) siRNA. Total RNA and protein was harvested at 48 h after transfection. Upper panel: TWIST1 mRNA expression was examined using sqRT-PCR. Data are presented as mean ± SD (n=3) relative to the levels of LNCaP cells transfected with a non-silencing control siRNA. A paired t-test was performed and a two-tailed p value <0,05 is indicated with a *. Lower panel: Representative Western blots of TWIST1, AR, NKX3-1 and α- tubulin (loading control) are shown.