Figure 2

A Expression of ERα in MCF-7S, MCF-7 ATCC, and MCF-7 NKI. Serum-starved MCF-7S cells were grown in 6-wells plates, and were lysed. Levels of ERα were analyzed by Western blotting. B Induction of cyclin D1 gene expression in MCF-7S, MCF-7 ATCC, and MCF-7 NKI by E2. Serum-starved MCF-7S cells were grown in 6-wells plates, and were given no mitogens (-), or 1 nM E2 (E2). The cells were lysed after 6 h. Levels of cyclin D1 were analyzed by Western blotting.